pooled sera meaning in English
混合血清
Examples
- Generally , the overall researches can be summarized to the following phases : 1 . ak auto ab was purified from human pooled sera by affinity chromatography
我们实验室对akautoah进行了广泛深入的研究，主要研究分为以下几个阶段： 1 - After absorbed with protein g - sepharose beads to remove the igg antibody , the pooled sera were then used for immunoscreening of the cdna library of schistosoma japonicum adult worms
蛋白同源性分析显示， sj43b编码蛋白与来自人和、鼠的含洲3结构‘域蛋白的同源性为3洲。 - Then , the gene was cloned into vector pgem - t and subcloncd into expression vector pgex - 6p - l and induced by iptg . western blot analysis showed that the fusion protein expressed could be recognized by the specific ige antibody in the pooled sera
0mmoul的iptg浓度、 15c 37c诱导表达温度和lh 411诱导表达时间范围内，改变相应条件对重组蛋白的可溶性表达没有明显影响。 - The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed . the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold . in order to further analyse the epitope features of the protein relevant to the specific ige antibody , the phage display library of random peptides was screened by pooled sera with the specific ige antibody
借助现行生物信息学分析手段，通过互联网进入genbank数据库，对sj43b与已知序列进行同源性比对，应用dnatoois软件对其编码蛋白序列的氨基酸纽成、亲水性、抗原性和可及性进行分析，并在blastp程序下在genbank蛋白数据库中对sj43b编码3人卞医科大学博士学杠论文蛋白进行同源性检索。